Enzyme Activity Calculator

User Guide

1. Enter Substrate Concentration ([S]): Input the substrate concentration in millimolar (mM). For example, enter 5 if the concentration is 5 mM.
2. Enter Enzyme Concentration ([E]): Provide the enzyme concentration in micromolar (µM). For example, enter 2 if the concentration is 2 µM.
3. Enter Reaction Time: Specify the reaction time in minutes. For example, enter 10 for a 10-minute reaction.
4. Enter Change in Product Concentration: Input the change in product concentration in micromolar (µM). For example, enter 20 if the change is 20 µM.

Formula

The formula to calculate enzyme activity is:

$\text{Enzyme Activity}=\frac{\mathrm{\Delta }\text{Product Concentration}}{\text{Reaction Time}\times \text{Enzyme Concentration}}$

Calculation Examples

Example 1

• Substrate Concentration ([S]): 10 mM
• Enzyme Concentration ([E]): 5 µM
• Reaction Time: 15 minutes
• Change in Product Concentration: 30 µM

Calculation:

Calculate Enzyme Activity:

$$\text{Enzyme Activity}=\frac{30}{15\times 5}=\frac{30}{75}=0.40\text{µM/min/µM enzym}$$

Example 2

• Substrate Concentration ([S]): 8 mM
• Enzyme Concentration ([E]): 3 µM
• Reaction Time: 20 minutes
• Change in Product Concentration: 24 µM

Calculation:

$$\text{Enzyme Activity}=\frac{24}{20\times 3}=\frac{24}{60}=0.40\text{µM/min/µM enzyme}$$

I hope you liked our Enzyme Activity Calculator and found it useful for your project. How was your experience using the tool? If you face any errors, please report them on our “Report an Error” page.