Input any two values—Absorbance, Molar Concentration, or Extinction Coefficient—to determine the missing parameter. The calculation assumes a path length (l) of 1 cm.
The Extinction Coefficient Calculator is an essential tool for determining the relationship between absorbance, molar concentration, and the extinction coefficient of a substance.
It is widely used in biochemistry, spectroscopy, and molecular biology to calculate protein concentration, verify solution purity, or analyze chemical reactions. This calculator simplifies the process with step-by-step explanations, ensuring accurate results.
Formula
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Absorbance (A):
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Molar Concentration (c):
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Extinction Coefficient (ε):
Here, is the path length, typically 1 cm.
Solved Examples
Example 1: Calculate Absorbance
Step | Value | Explanation |
---|---|---|
Molar Concentration (c) | 0.02 mol/L | Input value |
Extinction Coefficient (ε) | 15,000 L/mol·cm | Input value |
Formula | A=ε⋅c⋅l | Apply formula |
Calculation | Absorbance is 300 |
Example 2: Calculate Extinction Coefficient
Step | Value | Explanation |
---|---|---|
Absorbance (A) | 1.5 | Input value |
Molar Concentration (c) | 0.001 mol/L | Input value |
Formula | | Apply formula |
Calculation | Extinction Coefficient is 1,500 L/mol·cm |
Applications
- Protein Extinction Coefficient Calculator: Estimate the extinction coefficient for protein analysis.
- Protein Concentration Calculator: Derive protein concentration using absorbance and known extinction coefficients.
- Spectroscopy Analysis: Simplify the calculation of absorbance or molar concentration in chemical studies.
- Chemical Reaction Analysis: Use the extinction coefficient to study reaction kinetics.
FAQs
How do I calculate absorbance?
Use the formula:
where ε is the extinction coefficient, c is the molar concentration, and l is the path length.
What is the extinction coefficient formula?
The extinction coefficient (ε) is calculated as:
How is molar concentration calculated?
To calculate molar concentration, use:
Can this be used for protein concentration?
Yes, the calculator can be applied to determine protein concentrations when the extinction coefficient is known.
Calculate absorbance, molar concentration, or extinction coefficient with the Extinction Coefficient Calculator. Ideal for spectroscopy, protein concentration, and chemical analysis with step-by-step calculations.